S. Abd Hakim, Eva Marlina Ginting, Alkhafi Maas Siregar, Rugaya, Abd Rais
The spectrum pattern analysis of the urease enzyme immobilization technique in PVA, a PVA polymer that is soluble in warm water, has been carried out. There are differences in the spectrum patterns of PVA, PVA-KTpClPB and PVA-Enzyme-KTpClPB solutions, respectively UVA, UVB and UVC. The purpose of this study was to create a cholesterol sensor whose analyte cholesterol on the indicator electrode was coated with a PVA-Enzyme cholesterol (ChOx)/GA 2.9%/PANI-p-toluenesulfonic acid/PVC-KTpClPB-o-NPOE 61% membrane. The method used is the potentiometric biosensor method of ChOx enzyme immobilization technique in PVA, which is symbolized by (PVA-E ChOx). This study was conducted by comparing the indicator electrode membrane coated with PVA-variation of Enzyme cholesterol (ChOx)/GA 2.9%/PANI-benzenesulfonic acid/PVC-KTpClPB-o-NPOE 61%. ChOx enzyme was varied at 1:1 and 1:3. The results of the UV-Vis analysis of ChOx enzyme immobilization in PVA obtained a spectrum pattern that was the same as the ChOx spectrum pattern on the ChOx enzyme 1:1. After obtaining the best results from the PVA-E ChOx membrane at 1:1. Continued coating of the first indicator electrode GA 2.9% / PANI-p-toluenesulfonic acid / PVC-KTpClPB-o-NPOE 61%. The second electrode GA 2.9% / PANI-benzenesulfonic acid / PVC-KTpClPB-o-NPOE 61%. Both membranes were analyzed by EDX, XRD, and FTIR, each placed on a tungsten wire and glass preparation, to determine the best membrane. The best membrane is PVA-E1 ChOx / GA 2.9% / PANI-p-toluenesulfonic acid / PVC-KTpClPB-o-NPOE 61%. © 2025, Rasayan Journal of Chemistry, c/o Dr. Pratima Sharma. All rights reserved.
Department of Physics, Universitas Negeri Medan, Jalan Willem Iskandar, Pasar V, Medan, 20221, Indonesia; Department of Chemistry, Universitas Negeri Medan, Jalan Willem Iskandar, Pasar V, Medan, 20221, Indonesia
