Rini Hafzari, Annisa, Anita Kairani, Muchamad Nur Cholis, Listya Puspa Kirana, Nurul Huda Panggabean, Nurbaity Situmorang, Dwi Ratna Anjaning Kusuma Marpaung
The adulteration and mislabeling of food ingredients, particularly meat products, pose a significant concern for consumers. In order to address this issue, it is crucial to use precise and sensitive methods for species identification, mainly when dealing with samples that contain a limited number of copies of DNA. The primary objective of this study is to design a digital nanoplate polymerase chain reaction (PCR) technique for the purpose of quantifying species identification. The primer used in this study was cytochrome b gene. The results showed that target DNA can be amplified using the digital PCR nanoplate until the lowest sample concentration of 0.0013 ng/µL. This study can be applied to enhance species identification processes in food samples by utilizing digital PCR nanoplates. © (2024), (Journal of Microbiology). All rights reserved.
Medan State University, Faculty of Mathematics and Sciences, Department of Biology, William Iskandar PS. V. Medan, Indonesia; Padjadjaran University, Faculty of Mathematics and Sciences, Department of Biology, Jl. Raya Bandung Sumedang KM.21, Jatinangor, Indonesia; PT. Genecraftlabs, Bussiness Park Kb, Jeruk, Jl. Meruya Ilir Raya No.8., Jakarta, Indonesia
