A Comparative Study of Fresh Blood and Probio FM–Fermented Tofu Waste as Growth Media for Black Soldier Fly (Hermetia illucens) and Their Application in Laying Quail Feed

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Muhammad Rido, Montesqrit, Harnentis, Fajri Maulana, Satri Yusasra Agasi, Fadhli Fajri, Heppy Setya Prima, Malikil Kudus Susalam, Putri Damela

2026 Journal of Animal Health and Production Vol. 14 Issue 2 Article Cited by 0

Abstract

This study aimed to determine the optimal growth media formulation and Probio FM utilization level to produce Black Soldier Fly (BSF) maggot meal with the best nutritional quality, as well as to evaluate the effect of BSF maggot meal inclusion in laying quail diets on production performance. The research consisted of two experimental stages. The first stage investigated the interaction between fresh blood and fermented tofu waste ratios combined with different Probio FM levels as growth media for BSF larvae. A Completely Randomized Design (CRD) in a factorial pattern was applied, consisting of two factors and three replications. Factor A was the ratio of fresh blood to tofu waste (1:2, 1:3, and 1:4), and Factor B was the Probio FM level (25, 50, and 75 ml/kg of growth media). The parameters measured were dry matter, crude protein, and crude fat contents of BSF maggot meal. The results showed no significant interaction between the blood-to-tofu waste ratio and Probio FM level on dry matter, crude protein, or crude fat contents. The second stage employed a CRD with five dietary treatments: A (control diet without BSF maggot meal), B (diet with 1.5% BSF maggot meal), C (3%), D (4.5%), and E (6%), each with four replications. The analysis of variance indicated that the inclusion of BSF maggot meal had no significant effect (P>0.05) on feed intake and egg weight, but had a highly significant effect (P<0.01) on egg mass, egg production, and feed conversion ratio (FCR). In conclusion, the ratio of fresh blood to tofu waste at 1:4 combined with 25 ml/kg Probio FM produced BSF maggot meal with high dry matter, high crude protein, and optimal crude fat content. Furthermore, inclusion of 6% BSF maggot meal in the diet maintained the production performance of laying quails, with feed consumption of 22.90 g/head/day, quail-day egg production of 72.53%, egg weight of 10.13 g/egg, egg mass of 7.35 g/egg, and a feed conversion ratio of 3.15. © 2026 by the authors.

Affiliations

Department of Animal Science, Faculty of Agriculture, Universitas Lambung Mangkurat, Banjarbaru, Indonesia; Department of Animal Nutrition and Feed Technology, Faculty of Animal Science, Universitas Andalas, 25163, Indonesia; Program Studi Teknologi Pakan Ternak, Jurusan Teknologi Industri Pertanian, Politeknik Negeri Tan-ah Laut, Tanah Laut, 70815, Indonesia; Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Medan, North Sumatra, Medan, Indonesia; Department of Agroindustry, Study Program of Animal Science, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang, Sijunjung, Indonesia; Department of Animal Science, Faculty of Agriculture, Universitas Teuku Umar, Aceh, Meulaboh, Indonesia