Melva Silitonga, Hendro Pranoto, Adriana D. Lumbangaol, Erlintan Sinaga, Feimmy R P. Sipahutar, Fajar A. Sinaga
Bidens pilosa leaves ethanol extract (EBP) has been evaluated for its anti-inflammatory, antimycobacterium, and immunomodulatory properties. However, the potential of EBP against colon injury in alcoholic rats remains elusive. In this regard, the potential EBP was investigated for its anti-cancer effect in alcohol-induced colon injury. The EBP with three different doses was treated for six weeks in the alcohol animals. Blood level and colon tissues were examined in alcoholinduced colon. The modern pharmacological analysis was conducted to prove the potential of EBP against colon carcinogenesis. The results defined that alcohol administration significantly disrupted the goblet and crypt cells in the colon tissue. Alcohol decreased the blood parameters; notably, an MCV level of 52.68 ± 1.35 is lower than the control group at 55.5 ± 4.43 (P < 0.05). In the alcohol model, EBP supplementation (500 and 750 mg/kg) improved the colon histological damage and restored the blood parameters. The bioinformatic studies exhibited that the EBP bioactive compounds regulated the signaling pathway of main targets, notably CASP3, EGFR, CCL2, and STAT3, in the alcohol-induced colon carcinogenesis. These results are considered to be the underlying reference to investigate the potential EBP against alcohol-induced colorectal injury. © 2025 Silitonga et al.
Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Medan, North Sumatra, Indonesia; Bioinformatics Research Group, Department of Chemistry, Faculty of Mathematics and Natural Science, Universitas Indonesia, Depok, Indonesia; Department of Sports Studies, Universitas Negeri Medan, North Sumatra, Indonesia
