Diky Setya Diningrat, Adelia Febriyossa, Selvia Dewi Pohan, Heppy Setya Prima, Larasati Arum Utami, Eka Dodi Suryanto, Bagoes Maulana, Ayu Nirmala Sari, Asmin
Microtuber production through tissue culture offers an effective solution for producing high-quality, disease-free potato seed tubers. This study investigates the effects of light treatment and sucrose concentration on microtuber production in red and Granola potato varieties. The research was conducted at the Tissue Culture Laboratory of PT. G10 Agrotechnology in Medan, North Sumatra. The treatments applied were lighting regulation (bright with 1000 lux LED lighting for 24 hr vs. total darkness (0 lux, culture bottles wrapped with aluminum foil) in the incubation room and different sucrose concentrations (80, 100 & 120 g/L) added to the MS (Murashige & Skoog) basic medium. This research used a Completely Randomized Design method with 5 replications. Data were analyzed using ANOVA. Results showed that bright light treatment and 80 g/L sucrose concentration provided the best results for Granola and red potato plantlet growth from 14 to 70 days after planting. For the number, weight, and diameter of microtubers, the combination of dark light treatment and 120 g/L sucrose concentration produced the highest average values for both Granola and red potatoes. Overall, the growth and production of Granola potatoes were higher than those of red potatoes in every parameter. The results of this research can be used as a reference for producing red potato and Granola microtubers through tissue culture. © 2026 Malaysian Society of Applied Biology.
Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Medan, 20221, Indonesia; Department of Electrical Engineering, Faculty of Engineering, Universitas Negeri Medan, 20221, Indonesia; Department of Biology, Faculty of Sciences and Technology, Universitas Islam Negeri Ar-Raniry Banda Aceh, Indonesia; Department of Mathematics, Faculty of Mathematics and Natural Sciences, Universitas Negeri Medan, 20221, Indonesia
