Hendro Pranoto, Ivan I.L. Gaol, Meida Nugrahalia
This study aimed to examine the antioxidant activity and identify bioactive compounds present in the stem bark extract of Bischofia javanica. Samples were collected from Beringin Park, Jenderal Sudirman Street, North Sumatra Province, Indonesia. The extraction method employed in this research was maceration, utilizing three different solvents. The antioxidant activity was evaluated using the DPPH (2,2-Diphenyl-1-picrylhydrazyl) assay, while the identification of bioactive compounds was performed using GC-MS (Gas Chromatography-Mass Spectrometry) analysis. The results demonstrated varying levels of antioxidant activity in the methanol, ethyl acetate, and n-hexane extracts of B. javanica stem bark. The antioxidant activity was quantified by determining the IC50 value (inhibitory concentration at 50%). The IC50 values for the methanol, ethyl acetate, and n-hexane extracts were 31 µg/ml (strong), 467.09 µg/ml (weak), and 1521.69 µg/ml (weak), respectively. Furthermore, the study identified a total of 91 bioactive compounds, with 16, 34, and 41 compounds identified in the methanol, ethyl acetate, and n-hexane extracts, respectively. Based on the research findings, it can be concluded that the methanol extract of B. javanica stem bark exhibited potential as an antioxidant. © Biharean Biologist, Oradea, Romania, 2025
Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Medan, Jalan Willem Iskandar Pasar, V Medan, Indonesia
