Manihar Situmorang, J. Justin Gooding, D. Brynn Hibbert, Donald Barnett
A biosensor was constructed by covalently attaching pyruvate oxidase at the surface of electrodeposited polytyramine. The resulting biosensor is sensitive to pyruvate with a linear calibration plot in the concentration range of 0.1-3.0 mM pyruvate, slope 0.42 μA mM-1 and the detection limit was 0.05 mM pyruvate (S/N = 3). The use of polytyramine has shown to produce a stable biosensor with excellent reproducibility.
School of Chemistry, University of New South Wales, Sydney, NSW 2052, Australia; Department of Chemistry, FPMIPA, Universitas Negeri Medan, Sumatra Utara 20222, Jl. Willem Iskandar Ps. V. Medan, Indonesia
