Juwitaningsih Tita, Jahro Iis Siti, Sari Sri Adelila, Rukayadi Yaya
The necessity for new antibiotic compounds is becoming urgent, given the increasing number of bacteria that are resistant to one or several antibiotics. This study was aimed to assess the potential of 24 North Sumatra, Indonesia medicinal plants as a source of antibacterial compounds. Maceration with acetone solvents was used to extract the content from the medicinal plant samples. Then, antibacterial activity tests were conducted via in vitro on 6 pathogenic bacteria. The antibacterial screening was carried out using the M02-A11 Clinical and Laboratory Standard Institute (ClSI) diffusion method followed by the determination of Minimum Inhibitory Concentration (MIC) with Microdilution Methods M07-A9 (CSSI) and determination of Minimum Bactericide Concentration (MBC). Phytochemical tests were carried out on extracts which showed high activity. The results confirmed that most of the samples showed antibacterial activity against 4 to 5 of test bacteria. Strong antimicrobial activity was shown by Phyllantus urinaria L extract against S. saprophyticus and Cassia angustifolia plant extracts against B. cereus and S. saprophyticus bacteria, each with a concentration of 312.5 ug/mL. The C. angustifolia is bacteriostatic, whereas P. urinaria L is bactericidal with an MBC value of 1250 ug/mL. Furthermore, P.urinaria contains alkaloids, flavanoids, terpenoids, saponins and tannins and so does C. Angustifolia. © 2020 World Research Association. All rights reserved.
Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Negeri Medan, Jl. Willem Iskandar Pasar V, Medan Estate, Medan, 20221, Indonesia; Lab. of Natural Products, Institute of Bioscience, Universiti Putra Malaysia, 43400UPM Serdang, Selangor Darul Ehsan, Malaysia
