Elly Sufriadi, Yuliani Aisyah, Fauziah Harahap, Yuliana Fernando, Vivi Mardina
Pogostemon cablin Benth var Tapak Tuan, Aceh, Indonesia is a plant herb potentially to be developing with culture method. However, culture of this cultivar (stem and leave of bud0 explants sampled from outdoor trees is sometimes challenges due to contamination not overcome by standard surface-sterilization. An Improve method, involving a 5-minute immersion of explants in 5% (v/v) detergent, 1% fungicide and bactericide, 5% NaClO and 0.5% antibioticswhich does not interfere with normal explant and callus development, is described in detail. © Published under licence by IOP Publishing Ltd.
Department of Chemistry, Faculty of Mathematical and Natural Science, Syiah Kuala University, Aceh, 2311, Indonesia; Department of Agriculture Product Technology, Faculty of Agriculture, Syiah Kuala University, Aceh, 2311, Indonesia; Department of Biology, Faculty of Mathematics and Natural Sciences, State University of Medan, Aceh, 2311, Indonesia; Department of Biology, Faculty of Engineering, Samudra University, Kampus Unsam Meurandeh, Langsa, 24415, Indonesia
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