Heppy Setya Prima, Rusfidra Rusfidra, Fatridha Yansen, Fajri Maulana, Mia Ayu Gusti
Background and Objective: The exploration of lactic acid bacteria in integration of specific halal certification is one of the major research topics in the fields of health, food, animal husbandry and agriculture. This study aimed to investigate antimicrobial potentials of probiotic lactic acid bacteria isolated from Rinuak fish (Psilopsis sp.) from Lake Maninjau, Indonesia. Material and Methods: Totally, 15 lactic acid bacteria were isolated from four samples of Rinuak fish (Psilopsis sp.) and investigated for their characteristics as probiotic candidates using conventional laboratory assessments and 16S rRNA sequencing methods.Results and Conclusion: Five isolates were identified as probiotic candidates, including IR2.2, IR2.4, IR4.1, IR4.3 and IR4.5 due to their good resistance of gastric pH ranging 84.24–88.01% and their survival ability against bile salts (resistance of 50.37–57.35%). The IR4.3 was identified to generate the greatest antimicrobial activity against Escherichia coli ATCC 0157, Staphylococcus aureus ATCC 25923 and Salmonella enteridis ATCC 13076 with their diameter of inhibition zone of 22.46, 19.34 and 9.41 mm, respectively. The 16S RRNA sequencing method verified that the lactic acid bacteria isolated from rinuak fish (Psilopsis sp.) included 97.69% similarity to Lactobacillus fermentum strain 4901. This strain promised as an antidiarrheal and antityphoid agent and a natural food preservative appropriate for incorporation into HALAL-compliant foods and pharmaceutical products. © (2025), (National Nutrition and Food Technology Research Institute). All rights reserved.
Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Medan, North Sumatra, Medan, Indonesia; Department of Animal Science, Faculty of Science, Universitas Andalas, West Sumatra, Padang, Indonesia; Department of Pharmacy, Faculty of Health Sciences, Universitas Sumatera Barat, West Sumatra, Pariaman, Indonesia; Department of Agricultural Industrial Technology, Study Program of Animal Feed Technology, Politeknik Negeri Tanah Laut, South Kalimantan, Indonesia; Department of Management, Faculty of Economics and Business, Universitas Negeri Padang, Padang City, Indonesia
