Ermila Hafni Nasution, Fauziyah Harahap, Hasruddin, Novita Wahyuni
Orchid plants are one of the most popular types of ornamental plants because of their beauty and diversity, so this plant is widely cultivated and has a high selling value. Cattleya sp. orchid propagation using seeds takes a long time, which is about 4 to 7 years, so we need other ways to overcome this. To preserve this plant, it needs to in vitro technology. In vitro technique is one of the propagation technologies that is a solution to solve the problem of lack of seeds, because it can provide seeds in large quantities, uniform and independent of the season. The aim of this research was to determine the best interaction of growth regulator naphthalene acetic acid (NAA), benzyl amino purine (BAP) and interaction of both on growth induction of Cattleya sp. Cytokinin interact with auxins in determining the direction of cell differentiation. The research method used a completely random design with various concentrations of naphthalene acetic acid (NAA: 0, 0,2, 0,4 ppm) and benzyl amino purine concentration (BAP: 0, 1, 2, 3 ppm). The parameter observations are number of buds, leaves and roots. The data were analyzed descriptively and inferentially with ANOVA. The result showed there is no significantly effect of NAA, BAP and their interaction on number of buds, number of leaver and number of roots. Within 12 weeks of observation the highest number of buds, leaves and roots was obtained from combination of 0,4 ppm NAA + 0 ppm BAP were 7, 25 and 10. The lowest number of buds, leaves and roots was obtained from combination 0 ppm NAA + 0 ppm BAP were 0, 5 and 1. © 2022 American Institute of Physics Inc.. All rights reserved.
Biology Education of Postgraduate School Universitas Negeri Medan, Jalan Willem Iskandar, Pasar V Medan Estate, North Sumatera, 20221, Indonesia; Departement of Biology, Faculty of Mathematics and Natural Science, Universitas Negeri Medan, Jalan Willem Iskandar, Pasar V, North Sumatera, 20221, Indonesia; Department of Chemistry Engineering, Faculty of Engineering, Universitas Sumatera Utara, Jalan Almamater, North Sumatera, 20155, Indonesia
