Selvia Dewi Pohan, Noor Liyana Sukiran, Jamsari Jamsari, Noraziyah Abdul Aziz Shamsudin
The 4-way-F1 rice lines were developed through the marker-Assisted breeding method, which used three parental lines: Pulau Batu (Sumatran local rice cultivar), Inpari 48 Blas, and IR64-Sub1. Pulau Batu is an outstanding local rice cultivar with excellent agronomic characteristics and yield. However, it still lacks resistance to blast diseases. The breeding process was conducted between ten individuals of each recipient and donor parent to create as many as 41 F1 hybrids. Reciprocal crossing was also carried out to observe the maternal effect of trait heritability from parent to offspring. After hybrid confirmation using PCR-based analysis, these F1 hybrids continued growing and crossed to develop the 4-way-F1 generation. DNA marker RM 302 was used as a background marker to identify hybrid plants due to its high level of polymorphism and its ability to differentiate each parent. As many as 164 of 4-way-F1 progenies resulted from the previous crossing; they continued to grow and underwent a selfing pollination. Phenotypic screening followed the SES-blast test standard, which showed most of the AB lines were categorized as resistant (56.5%), 28.3% as moderately resistant, and 15.2% as susceptible. Meanwhile, BA lines also showed an almost similar pattern, with most of the lines grouped into resistant (52.8%), 27.8% as moderately resistant, and 19.4% as susceptible. Agronomic evaluation of 4-way-F1 progenies showed that the AB and BA plants did not appear to differ significantly in agronomic characteristics, except for the DTF, TGW, and YLD. On average, AB plants flowered earlier than BA (p<0.001). Otherwise, BA plants had higher TGW and YLD than AB (p<0.05 and p<0.01, respectively). However, no significant differences between AB and BA plants were observed regarding PH, PTN, PL, NFG, and NUFG p<0.001). © Published under licence by IOP Publishing Ltd.
School of Biological Science and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, Selangor Darul Ehsan, UKM Bangi, 43600, Malaysia; Department of Biology, Faculty of Mathematics and Natural Science, State University of Medan, Jln. Willem Iskandar Pasar v Medan Estate, North Sumatra, Medan, 20221, Indonesia; Department of Agrotechnology, Faculty of Agriculture, Andalas University, West Sumatra, Padang, 25163, Indonesia; Natural History Museum, Universiti Kebangsaan Malaysia, Selangor Darul Ehsan, UKM Bangi, 43600, Malaysia
