Dna barcoding of andaliman (Zanthoxylum acanthopodium dc) from north sumatra province of indonesia using maturase k gene

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Cicik Suriani, Eko Prasetya, Tri Harsono, Johannes Manurung, Hary Prakasa, Dina Handayani, Miftahul Jannah, Yuanita Rachmawati

2021 Tropical Life Sciences Research Vol. 32 Issue 2 Article Cited by 6 Quartile

Abstract

Andaliman (Zanthoxylum acanthopodium DC) is a native plant of North Sumatra province. Zanthoxylum acanthopodium is a member of Rutaceae family widely found in northern Sumatra, Indonesia. The aim of this study was to barcode Z. acanthopodium in North Sumatra province, Indonesia based on cpDNA maturase K (matK). Samples were collected in seven localities across six regions of North Sumatra province. Phylogenetic analysis was conducted using Maximum Likelihood method. The results of phylogenetic analysis indicate that Z. acanthopodium is a monophyletic group that is derived from a common ancestor. The results of the phylogenetic tree construction show that there is a grouping of accession between Z. acanthopodium species separate from other species in the Zanthoxylum genus as well as those of the Rutaceae family. The results showed that cpDNA matK marker can effectively be used as DNA barcoding to identify Z. acanthopodium. © Penerbit Universiti Sains Malaysia, 2021.

Affiliations

Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Medan, Jl. Willem Iskandar, Pasar V, Medan Estate North Sumatra, Medan, 20221, Indonesia; Department of Biology, Faculty of Sciences and Technology, Islamic University of Assyafiiyah, Jl. Jatiwaringin Raya, No 12, East Jakarta, 17411, Indonesia; Department of Biology, Faculty of Sciences and Technology, Islamic State University Sunan Ampel Surabaya, Jl. Ahmad Yani 117 East Java, Surabaya, 60237, Indonesia