Siti Maulida, Kartini Eriani, Firman Muhammad Nur, Nur Fadli, Agung Setia Batubara, Abdullah Adan Muhammadar, Mohd Nor Siti-Azizah, Martin Wilkes, Zainal Abidin Muchlisin
Naleh fish Barbonymus sp. is a commercial freshwater fish, which is indigenous to Aceh, Indonesia. The population of this species has declined over the years as a result of habitat perturbations and overfishing. Hence, the crucial need to develop a cryopreservation method to support breeding programs. This involved the use of a cryoprotectant as an important component. The objective of this study, therefore, was to explore the best cryoprotectant for naleh fish spermatozoa, and a total of five types were tested. These include the DMSO, Methanol, Ethanol, Glycerol, and Ethylene Glycol at a similar concentration of 10%, which were individually combined with 15% egg yolk, and every treatment was performed in three replications. Conversely, Ringer’s solution was adopted as an extender, and the sperm was cryopreserved in liquid nitrogen for 15 days. The results showed significant influence on sperm motility and viability, as well as egg fertility of naleh fish (P <0.05), although the DMSO provided the best outcome, compared to others at 47.17%, 50.13%, and 45.67%, respectively. Furthermore, DNA fragmentation had not occurred in the fresh and cryopreserved sperm samples, indicating the protective effect of tested cryoprotectants. It is concluded that the 10% DMSO and 15% egg yolk is the best cryoprotectant for naleh fish spermatozoa. © 2021, Universidade de Sao Paulo. Faculdade de Medicina Veterinaria e Zootecnia. All rights reserved.
Universitas Syiah Kuala, Faculty of Mathematics and Natural Sciences, Master Program in Biology, Banda Aceh, Indonesia; Syiah Kuala, Faculty of Marine and Fisheries, Department of Aquaculture, Banda Aceh, Indonesia; Universitas Syiah Kuala, Mathematics and Sciences Application, Banda Aceh, Indonesia; Universitas Negeri Medan, Faculty of Mathematics and Natural Sciences, Medan, Indonesia; Universiti Malaysia Terengganu, Institute of Marine Biotechnology, Terengganu, Malaysia; Coventry University, Center for Agroecology, Water and Resilience, Coventry, United Kingdom; Universitas Syiah Kuala, Marine and Fisheries Research Center, Banda Aceh, Indonesia
